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SnRK2是一类植物特有的蛋白激酶，在植物ABA信号转导、抵御逆境胁迫和生长发育过程中发挥重要作用。为探究花生SnRK2基因家族的功能，通过生物信息学分析共在花生中鉴定到22个SnRK2基因家族成员，命名为AhSnRK2.1—AhSnRK2.22。系统进化分析表明这22个AhSnRK2s可分为3个聚类。蛋白质结构分析表明AhSnRK2.1除含有保守的S＿TKc结构域外，其C端还含有非生物胁迫响应结构域和ABA诱导调节结构域。基因表达模式分析显示，AhSnRK2.6在花生各种组织/器官中的相对表达较高，同时，干旱、ABA和SA处理也显著诱导其表达，表明AhSnRK2.6可能在花生胁迫应答过程中发挥重要作用。蛋白质互作网络分析揭示AhSnRK2s家族成员可能在磷酸化调控和ABA信号响应中具有潜在作用。以上结果为探究花生AhSnRK2s基因功能和开展抗逆遗传育种提供了基础。
Abstract：Sucrose non-fermenting-1-related protein kinase 2(SnRK2) is a class of plant-specific serine/threonine(Ser/Thr) protein kinases, which play an important role in ABA signal transduction, resistance to abiotic stresses, and plant development. To explore the functions of the SnRK2 gene family in peanut, a total of 22 members(named AhSnRK2.1 to AhSnRK2.22) of the SnRK2 gene family were identified in peanut through bioinformatics analysis. Phylogenetic analysis showed that the 22 AhSnRK2s were divided into three different clades. The analysis of protein structure showed that AhSnRK2.1 contains a conserved S＿TKc domain, an abiotic stress response domain, and an ABA-induced regulatory domain, respectively. Gene expression patterns analysis revealed that the expression level of AhSnRK2.6 in various tissues of peanut, as well as under the conditions of drought, ABA, and SA treatments, was higher than that of other AhSnRK2s. This indicates that AhSnRK2.6 may play a significant role in the stress response process of peanut. Protein-protein interaction analysis revealed the potential roles of the AhSnRK2s family in the two pathways of phosphorylation regulation and ABA signal response. The above results provide a basis for the functional analysis of the AhSnRK2s gene family and its application in stress-resistant genetic breeding.
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基本信息:
DOI：10.14001/j.issn.1002-4093.2025.04.001
中图分类号:S565.2
引用信息:
[1]冯兰兰,高蒙,王振宇.花生AhSnRK2基因家族鉴定及生物信息学分析[J].花生学报,2025,54(04):357-367.DOI:10.14001/j.issn.1002-4093.2025.04.001.
基金信息:
河南省博士后经费; 河南省农业科学院重大应急专项(2024ZJTH001); 河南省重大科技专项(201300111000)